Circligase ssdna ligase. Under standard .

Circligase ssdna ligase. circularisation) of ssDNA and ssRNA templates. Feb 14, 2014 · Applications Production of ssDNA templates for rolling-circle replication or rolling-circle transcription experiments. Linear ssDNAs and ssRNAs of greater than 30-35 bases (sequence dependent) are circularised by CircLigase ssDNA Ligase. 5], 10 mM KCl, 5 mM MgCl 2, and 1 mM DTT) were mixed and incubated for 1 h at 60 °C. After adaptor ligation, ssDNA is converted to dsDNA, followed by second adaptor tagging by T4 DNA ligase. The DNA Ligase Selection Chart helps you select the optimal DNA ligase for various applications. Linear ssDNAs and ssRNAs of greater than ~30 bases are circularised by CircLigase ssDNA Ligase. Introduction CircLigase II ssDNA Ligase* is a thermostable ligase that catalyses intramolecular ligation (i. Thermostable ligase that catalyzes the intramolecular ligation (i. DNA, RNA and hybrid substrates are represented and require specific enzymes to achieve the highest-efficiency ligation. circularization) of ssDNA templates having a 5 Note that TS2126 RNA ligase can be substi-tuted with CircLigase II ssDNA ligase from Lucigen (Middleton, WI). Standard reaction conditions produce no detectable single-stranded DNA concatamers or concatameric DNA circles. Under standard reaction conditions, virtually no linear concatamers CircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i. CircLigase™ ssDNA Ligase* is a thermostable ATP-dependent ligase that catalyses intramolecular ligation (i. It ligates the ends of ssDNA in the absence of a complementary sequence. Linear ssDNAs of greater than ~15 bases are circularized by CircLigase II ssDNA Ligase. CircLigase ssDNA Ligase 1 | Government Scientific Sourceby LUCIGEN CORP $223. Set up the following reaction in a sterile microfuge tube: 2. 5 pmol/μl miRNA-21-Padlock-CircLigase, 50 μM adenosine triphosphate, 2. , circularization) of single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA) substrates that have both a 5′-monophosphate and a 3′-hydroxyl group. Nov 19, 2012 · Protocol Set up the following reaction in a sterile microfuge tube: Components Volume ssDNA/RNA Substrate 20 pmol (1 pmol/ul) 5´ App DNA Oligonucleotide 200 pmol (10 pmol/µl) 10X NEBuffer 1 2 µl 50 mM MnCl 2 (for ssDNA ligation only) 2 µl Thermostable 5´ App DNA/RNA Ligase 2 µl (40 pmol) Nuclease-free Water to 20 µl Incubate at 65°C for 1 hour Aug 5, 2024 · After the simulation of the secondary structure for desired circular ssDNA, the linear ssDNA substrate is designed to have its ends on the duplex part (≥5 bp). circularization) of ssDNA templates having a 5-phosphate and a 3-hydroxyl group Nov 25, 2020 · In previous studies, T4 DNA ligase was used for single-stranded (ss) DNA library preparation [8] and branch ligation [9], and could be used instead of CircLigase due to its high efficiency and low bias; however, CircLigase can ligate the strand without requiring a double-stranded adaptor or intermediates. In contrast to T4 DNA Ligase and Ampligase ® DNA Ligase, which ligate DNA ends that are annealed adjacent to each other on a complementary DNA sequence, CircLigase ssDNA Ligase ligates ends of ssDNA . circularization) of ssDNA templates In this webinar, you will learn about our recently developed a single-molecule sequencing method to characterize end-joining ligase fidelity (discrimination against ligating mismatched overhangs) and bias (sequence preferences) for short cohesive ends in a high throughput manner. CircLigase™ ssDNA Ligase* is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group CircLigase ssDNA Ligase This thermostable, ATP-dependent ligase catalyses intramolecular ligation (i. Linear ssDNAs of greater than ~15 bases are circularized by CircLigase ssDNA Ligase. Lane 1. 1. Lucigen has an extensive portfolio of life science products including NGS kits, enzymes, competent cells, and cloning systems, targeting fast growth applications in clinical diagnostics, drug The method involved denaturing the linear, fragmented dsDNA to obtain linear ssDNA fragments, ligating the linear ssDNA with CIRCLIGASE™ ssDNA ligase to obtain single-stranded DNA circle, and then amplifying the single-stranded DNA circle using random primers and Phi29 DNA polymerase via RCA. BIOSEARCH TECHNOLOGIES INC (Lucigen) CircLigase II ssDNA Ligase, 5,000 U @ 100 U/LAn ATP-independent, non-catalytic thermostable ligase that catalyses the intramolecular ligation (i. Here, we design an intramolecular terminal hybridization strategy to program Lucigen merges with Biosearch Technologies, the comprehensive genomics portfolio from LGC LGC acquires Lucigen in 2018, strengthening its reagents offering to the genomics market Lucigen is a leading developer, manufacturer and supplier of molecular biology enzymes, reagents and kits. This ligase is utilized with ssDNA templates for rolling-circle replication or rolling-circle transcription experiments and for RNA polymerase and RNA polymerase inhibitor assays. e Applications Include: Production of ssDNA templates for rolling-circle replication or rolling-circle transcription experiments CircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i. For example, Meyer's group established a method based on CircLigase II 5, 6. May 22, 2019 · The unique features of RNA ligase and TdT described above led us to devise a novel combinatorial application of these two enzymes for ssDNA ligation. CircLigase ssDNA Ligase Jan 19, 2023 · a (a) T4 DNA ligase and (b) CircLigase-catalyzed intramolecular circularization of a ssDNA, both of which are inevitably accompanied by intermolecular ligation to produce linear ligated products. CircLigase™ ssDNA Ligase, 100 U/µL, 5,000 U Catalogue number: Add to favourites CL4115K The method involved denaturing the linear, fragmented dsDNA to obtain linear ssDNA fragments, ligating the linear ssDNA with CIRCLIGASE™ ssDNA ligase to obtain single-stranded DNA circle, and then amplifying the single- stranded DNA circle using random primers and Phi29 DNA polymerase via RCA. Under standard reaction CircLigase™ ssDNA Ligase* is a thermostable ATP-dependent ligase that catalyses intramolecular ligation (i. A pool of 60 nt acceptor oligonucleotides ('60N') were ligated to 10 pmol of a 3´biotinylated3´biotinylated donor oligonucleotide CircLigase™ II ssDNA Ligase是一个热稳定性酶，催化具有5'-磷酸和3'-羟基基团的单链DNA模板的分子内连接（即环化）。与T4 DNA Ligase和Ampligase® DNA Ligase不同（连接彼此相邻的互补的DNA序列的末端）CircLigase II ssDNA Ligase可以在没有互补序列存在的情况下连接单链DNA末端。 Therefore, a 1:1 stoichiometry of ligase to substrate is required to drive the ligation reaction to completion. circularization) of ssDNA templates. In The unique features of RNA ligase and TdT described above led us to devise a novel combinatorial application of these two enzymes for ssDNA ligation. CircLigase ssDNA Ligase is a specialized enzyme used in molecular biology applications. It can catalyze the cyclization of single-stranded DNA or RNA, and intermolecular ligation, but the efficiency is higher when using single-stranded DNA as substrate. Intramolecular Epicentre Productligation of single-stranded DNA ends containing 5'-phosphates and 3'-hydroxyl groups Gene Target Solutions distributes Epicentre Bio ‘s (Illumina company) DNA Ligases, including Ampligase Thermostable DNA Ligase, Circligase ssDNA Ligase, T4 DNA Ligase and E coli DNA Ligase within Australia. Production of ssDNA templates for RNA polymerase and RNA polymerase inhibitor assays. This enzyme ligates the template by the 5'phosphate group and the 3' hydroxyl group. ssDNA RNA环化连接酶为热稳定的DNA RNA连接酶，不依赖于ATP，可催化单链DNA或单链RNA的环化，也可催化其分子间连接，但其对单链 Furthermore, CircLigase (a DNA ligase) can directly connect the 5′ and 3′ ends of ssDNA [22, 23], but some secondary structures can decrease the efficiency (e. This thermostable ATP-dependent ligase catalyzes the intramolecular ligation of ssDNA molecules that have a 5′-phosphate and a 3′-hydroxyl group without the need for a complementary oligonucleotide. Under standard CircLigase™ ssDNA Ligase Applications Production of ssDNA templates for rolling-circle replication or rolling-circle transcription experiments. 3. AMP) to the 3′-end of ssDNA, then RNA ligase would use them as an efficient ‘connector’ to 5′-phosphorylated ODN. Indeed, the practical efficiency of CircLigase II is lower than that of random splint-based ssDNA ligation using T4 DNA ligase [6]. no. Circleligase II是一种热稳定性酶，可以在没有互补序列存在的情况下催化具有5'-磷酸基团和3'-羟基基团的单链DNA（ssDNA）/单链RNA (ssRNA)的自环化，该反应不依赖于ATP。 This chart provides our recommendation for a choice of ligase to use in a reaction, based upon the type of substrate present. Under standard reaction ATP-independent, non-catalytic thermostable ligase that catalyzes the intramolecular ligation (i. Jan 13, 2021 · 该技术不仅将CircLigase的催化DNA成环产率稳定地提升至75%以上，而且突破了CircLigase对于可环化的DNA片段的长度的限制，在几百碱基的长链DNA上都得到了验证。课题组同时利用CircLigase预制备的单链DNA环作为起始组装材料获得了高纯度的DNA拓扑嵌套结构。图2. Use of CircLigase can provide circular products from ss-DNA template for RCA; however its activity on ds-DNA template is unknown (7 Feb 14, 2014 · Applications Production of ssDNA templates for rolling-circle replication or rolling-circle transcription experiments. 16USD$ 1 510,16 CircLigase I ssDNA ligase is a thermostable, single-stranded DNA (ssDNA) ligase that catalyzes the formation of phosphodiester bonds between the 5'-phosphate and 3'-hydroxyl termini of ssDNA fragments. In addition to its activity on ssDNA, CircLigase II enzyme also has activity in ligating a single-stranded nucleic acid having a 3'-hydroxyl ribonucleotide and a 5'-phosphorylated ribonucleotide or deoxyribonucleotide. Under standard reaction conditions Thermostable enzyme that catalyzes intramolecular ligation (i. CircLigase™ II ssDNA Ligase——单链DNA环化连接酶技术优势大于15个碱基的线性单链DNA，包括cDNA，均可被CircLigase II enzyme环化；标准反应条件下，不产生线性或环状的嵌合体结构；除了对单链DNA具有活性，CircLigase II也在具有3'-羟基核糖核苷酸和5'-磷酸化的核糖核苷酸或脱氧核糖核苷酸的单链核酸的连接 Article CircLigase ssDNA Ligase, 100 U/uL Product Number CL4111K Supplier Lucigen Size 1000 U Category Genomics & NGS Product Group NGS Enzymes Shipping Temperature DRY ICE Supplier URL Visit supplier product page Mar 14, 2013 · CircLigase II ssDNA ligase (Epicentre, cat. CL9025K), including 10× reaction buffer and 50 mM MnCl 2 solution PEG-4000 solution, 50% (wt/vol) (Sigma-Aldrich, cat. By using this permuted substrate with 5'-phosphate, the splint-free circularization is simply carried out by T4 DNA ligase. 4. Library insert size is critical to success using the TIRF Transformer system and rolling Description This thermostable ligase catalyses intramolecular ligation (i. The enzyme is therefore useful for making circular ssDNA molecules from linear ssDNA. Although RNA ligase can connect two ssDNA molecules, the reaction CircLigase™ ssDNA Ligase CircLigase™ssDNALigase应用产生单链DNA模板用于滚环复制或者滚环转录实验。产生单链DNA模板用于RNA聚合酶和RNA聚合酶抑制剂测定。CircLigase™IIssDNALigase是一个热稳定性酶，催化具有5'-磷酸和3'-羟基基团的单链DNA模板的分子内连接（即环化）。与T4DNALigase和Ampligase®DNALigase不同（连接 CircLigase™ Ⅱ ssDNA Ligase*は、5’リン酸基および3’ヒドロキシル基を持つssDNAテンプレートの分子内ライゲーション（環状化）を触媒する耐熱性酵素です。 Dec 31, 2020 · Circular single-stranded (ss) DNA is an essential element in rolling circle amplification and many DNA nanotechnology constructions. e. CircLigase ssDNA Ligase, 100 U/uL Lucigen CL4115K NGS Enzymes Circularize ssDNA templates CircLigase ssDNA Ligase* is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i. The enzyme is therefore useful for making circular ssDNA molecules from linear ssDNA Epicentre Biotechnologies offers a new enzyme called CircLigaseTM that circularizes single stranded DNA. CircLigase™ II ssDNA Ligase* is a thermostable enzyme that catalyzes intramolecular ligation (i. In the current study, we optimized the codon usage of the protein, and successfully expressed and purified a moderately thermostable ssDNA ligase. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group Jan 26, 2021 · Circular single-stranded (ss) DNA is an essential element in rolling circle amplification and many DNA nanotechnology constructions. Jan 1, 2011 · In this section, the circularization of ssDNA oligonucleotide molecules with the enzyme CircLigase ssDNA ligase (Epicentre) is described. Product information CircLigase™ ssDNA Ligase* is a thermostable ATP-dependent ligase that catalyses intramolecular ligation (i. It is commonly synthesized from linear ssDNA by a ligase, which nevertheless suffers from low and inconsistent efficiency due to the simultaneous formation of concatem … CircLigase™ II ssDNA Ligase* is a thermostable enzyme that catalyses intramolecular ligation (i. However, currently available enzymes exhibit only limited activity. Size: EACH (1,000 U) $ 1 510,161510. Incubate at 60°C for 1 hours or overnight. Bioz Stars score, Techniques, Protocol Conditions and more for Circligase Ssdna Ligase, supplied by Epicentre Biotechnologies. Oligonucleotides can be ordered from IDT, resuspended, and used directly in CircLigase reaction to produce synthetic library. It is commonly synthesized from linear ssDNA by a ligase, which nevertheless suffers from low and inconsistent efficiency due to the simultaneous formation of concatemeric byproducts. circularization) of ssDNA templates having a 5-phosphate and a 3-hydroxyl group Introduction CircLigase ssDNA Ligase† is a thermostable ATP-dependent ligase that catalyses intramolecular ligation (i. In addition, the Properties of DNA and RNA Ligases Jan 15, 2024 · A thermostable RNA ligase from bacteriophage TS2126 of Thermus scotoductus exhibits good ssDNA ligation properties [19]. In contrast to other ligases, CircLigase ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence. 00/KT Average lead time: Call for Lead Time ADD TO CART Add to Favorites + Create List Glass ware (21) Herzog Lab (20) Bottles (23) Bottles (20) Bottles (20) yXHwZqxvNak (17) WSaKvQcpsAG (18) TuLeuihdVztvKF (8) hbsEU (20) fFTKtVMpQxq (17) Favorites (13) freezer tape (10) Protocol for the CircLigase II ligation (CircLigase II ssDNA ligase, Epicentre CL9021) 1. The aim of this study was to express and purify a ligase that is highly active in aptamer circularization and to develop an efficient ligation strategy to solve the issues mentioned above. In contrast to T4 DNA Ligase and Ampligase™ DNA Ligase, which ligate DNA ends that are annealed adjacent to each other on a complementary DNA sequence, CircLigase ssDNA Ligase Introduction CircLigase ssDNA Ligase* is a thermostable ATP-dependent ligase that catalyses intramolecular ligation (i. 9 kb. ssDNA/RNA CircLigase is a thermostable DNA/RNA ligase, which is independent of ATP. CircLigase™ II ssDNA Ligase is a thermostable enzyme that catalyzes intramolecular ligation (i. By using this permuted substrate with 5′-phosphate, the splint-free circularization is simply carried out by T4 DNA ligase. Circular DNA molecules have enhanced chemical s CircLigase™ II ssDNA Ligase* is a thermostable enzyme that catalyses intramolecular ligation (i. Under 1. BIOSEARCH TECHNOLOGIES INC (Lucigen) CircLigase II ssDNA Ligase, 1,000 U @ 100 U/LAn ATP-independent, non-catalytic thermostable ligase that catalyses the intramolecular ligation (i. , circularization) of single-stranded DNA (ssDNA) templates having a 5`-phosphate and a 3`-hydroxyl group 1. ssDNA ligase for cloning and library construction. Data for Circligase Ssdna Ligase gathered from related PubMed articles. The reaction was incubated at 37 °C for 15 min, 65 °C for 30 min, and 95 °C for 5 min. Here, we identified an enzyme with strong ssDNA ligation activity upon searching the databases for proteins homologous to TS2126 RNA ligase, the known enzyme with the highest yet limited CircLigase™ ssDNA Ligase*は、5’リン酸基および3’ヒドロキシル基を持つssDNAテンプレートの分子内ライゲーション（環状化）を触媒する耐熱性のATP依存性リガーゼです。 The second method is based on ssDNA ligation catalyzed by RNA ligase, which joins a 5′-phosphorylated adaptor to the 3′-end of ssDNA. Intramolecular Epicentre Productligation of single-stranded DNA ends containing 5'-phosphates and 3'-hydroxyl groups Mar 27, 2023 · The 20 μl of reaction was carried out according to the instructions. Single-stranded DNA ligation with T4 DNA ligase and CircLigase . 5 mM MnCl 2, 5 U/μl CircLigase ssDNA Ligase, and 1× CircLigase reaction buffer (50 mM Mops [pH 7. Nov 10, 2016 · CircLigase™ ssDNA ligase (Epicentre, Madison, WI, USA) effectively catalyzes the circularization of single-stranded DNA and RNA by intramolecular ligation of a phosphate at the 5′ end and a hydroxyl group at the 3′ end without the use of “splint” oligonucleotide (Fig. , circularisation) of single-stranded DNA (ssDNA) and single- stranded RNA (ssRNA) substrates that have both a 5′-monophosphate and a 3′-hydroxyl group. , circularization) of single-stranded DNA (ssDNA) substrates that have both a 5′-monophosphate and a 3′-hydroxyl group. CircLigase II ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence. 1a). We reasoned that if TdT attached a few ribonucleotides (e. (c) Proposed ligatable DNA aptamer for the T4 DNA ligase, which also serves as a substrate that favors intramolecular circularization by the ligase. , circularisation) of ssDNA templates >30 nt having a 5′ phosphate and a 3′ hydroxyl group. circularisation) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group. circularisation) of single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA) substrates that have both a 5′-monophosphate and a 3′-hydroxyl group. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl 1. Under standard reaction Circle ligase ssDNA ligase and Circle ligase II ssDNA ligase from Lucigen. Apr 15, 2013 · Results and discussion ssDNA ligation using Circligase I and T4 RNA ligase I To assay for ligation, we designed two ssDNA oligonucleotides, referred to as “acceptor” and “donor” (Fig. Linear ssDNAs and ssRNAs of greater than ~30 bases are circularized by CircLigase ssDNA Ligase. , circularization) of ssDNA templates having a 5′-phosphate and a 3′-hydroxyl group. CircLigase II ssDNA Ligase is an improved version of our original CircLigase enzyme. This method allows determination of the relative frequency of all ligation products with or without mismatches, the ATP-independent, non-catalytic thermostable ligase that catalyzes the intramolecular ligation (i. Mar 3, 2009 · CircLigase™ II ssDNA Ligase is designed to catalyze the circularization of single-stranded DNA greater than 15 bases without the need for oligo bridges. 1 A). Introduction CircLigaseTM ssDNA Ligase† is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i. Aug 18, 2024 · After the simulation of the secondary structure for desired circular ssDNA, the linear ssDNA substrate is designed to have its ends on the duplex part (≥5 bp). DNA ligase is a specific type of enzyme that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. (A) CircLigase II ssDNA ligase converted 80 nt linear ssDNA oligos into circular ssDNA. circularization) of ssDNA templates Intramolecular ligation of single-stranded DNA ends containing 5'-phosphates and 3'-hydroxyl groups ATP-dependent, catalytic enzyme Circularizes ssDNA >30 bases long Compatible with high temperature ligation reactions due to thermostability of CircLigase™ enzyme Jan 24, 2017 · A simplified method of single-stranded library preparation To evaluate if splinted single-stranded ligation with T4 DNA ligase can substitute CircLigase in single-stranded library preparation, we created libraries with both ligation strategies using a DNA extract of a >50 000 year-old cave bear. Due to the high degree of adenylation CircLigase™ II ssDNA Ligase CircLigase™IIssDNALigase应用产生单链DNA模板用于滚环复制或者滚环转录实验。产生单链DNA模板用于RNA聚合酶和RNA聚合酶抑制剂测定。CircLigase™IIssDNALigase是一个热稳定性酶，催化具有5'-磷酸和3'-羟基基团的单链DNA模板的分子内连接（即环化）。与T4DNALigase和Ampligase®DNALigase不同 In contrast to T4 DNA Ligase and Ampligase ® DNA Ligase, which ligate DNA ends that are annealed adjacent to each other on a complementary DNA sequence, CircLigase II ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence. CircLigase ssDNA Ligase A thermostable ligase that catalyses the intramolecular ligation (i. g. Under standard reaction conditions, virtually no linear 1. It is suitable for various molecular biology applications, including circularization of ssDNA. CircLigase II ssDNA Ligase An ATP-independent, non-catalytic thermostable ligase that catalyses the intramolecular ligation (i. LGC acquires Lucigen in 2018, strengthening its reagents offering to the genomics market Lucigen is a leading developer, manufacturer and supplier of molecular biology enzymes, reagents and kits. circularization) of ssDNA templates having a 5´-phosphate and a 3´-hydroxyl group. pUC19 DNA/MspI (HpaII) Marker (Thermo Scientific); Lane 2. , circularisation) of single-stranded DNA (ssDNA) and single-stranded RNA (ssRNA) substrates that have both a 5′-monophosphate and a 3′-hydroxyl group. Lucigen has an extensive portfolio of life science products including NGS kits, enzymes, competent cells, and CircLigase II ssDNA Ligase * is a thermostable ligase that catalyses intramolecular ligation (i. Introduction This protocol details methods to prepare circular single stranded DNA library using 5’ phosphorylated synthetic linear ssDNA as starting material (synthetic library). Jan 18, 2023 · A new design of splint strands allows for the near-quantitative monomeric cyclization of single DNA strands as short as 16 bases long by DNA T4 ligase. CircLigase™ ssDNA Ligase and CircLigase™ II ssDNA Ligase are thermostable ATP-dependent ligases that catalyze intramolecular ligation (i. Nov 25, 2020 · In previous studies, T4 DNA ligase was used for single-stranded (ss) DNA library preparation [8] and branch ligation [9], and could be used instead of CircLigase due to its high efficiency and low bias; however, CircLigase can ligate the strand without requiring a double-stranded adaptor or intermediates. 89782-100ML-F) CircLigase™ II ssDNA Ligase* is a thermostable enzyme that catalyses intramolecular ligation (i. Real Time Stock Check ITEM #: 21849182 CircLigase* ssDNA Ligase 1, 000 Units^ CircLigase* ssDNA Ligase, Thermostable ligase that catalyzes the intramolecular ligation of ssDNA templates, ATP-dependent, catalytic enzyme, Circularizes ssDNA >30 bases long, Compatible with high temperature ligatio This listing is for Each CircLigase™ ssDNA Ligase* is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i. CircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i. However, its efficiency in library preparation from ssDNA was low or limited to only short ssDNAs. 80 nt linear ssDNA oligos (upper band Details Thermostable ligase that catalyzes the intramolecular ligation (i. , when the two ends were impeded to come close, the circularization yield decreased to only 26% [22]), and the specially engineered CircLigase is relatively expensive. Introduction CircLigaseTM II ssDNA Ligase† is a thermostable ligase that catalyzes intramolecular ligation (i. It circularizes fragments larger than 30 nucleotides and up to 1. The 0. Under standard Aug 25, 2023 · Note that TS2126 RNA ligase can be substituted with CircLigase II ssDNA ligase from Lucigen (Middleton, WI). Jan 30, 2025 · Abstract An enzyme with strong single-stranded DNA (ssDNA) ligation activity would be advantageous for many molecular biology applications. Linear ssDNAs and ssRNAs of greater than 30-35 bases (sequence dependent) are circularised by CircLigase ssDNA Ligase Jan 15, 2024 · In addition, commercial CircLigase is expensive and therefore not feasible for large-scale research on the circularization of aptamers. Incubate at 80°C for 10 minutes to inactivate the enzyme. Its core function is to catalyze the formation of a phosphodiester bond between the 5' phosphate and 3' hydroxyl termini of single-stranded DNA molecules, enabling the covalent joining of DNA fragments. tv bpx dhc ub zwalt py huw mnv0sf vfzd 8su8